Abcam, ab301003, Human INPPL1 knockout A549 cell line

Size: 1000000Cells / vial
INPPL1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Disease:Carcinoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control
: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-INPPL1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
INPPL1 also known as SHIP-2 (Src homology 2 domain containing inositol 5-phosphatase 2) participates in the regulation of intracellular signaling pathways by dephosphorylating phosphatidylinositol 345-trisphosphate (PIP3). It acts as a phosphatase with a molecular mass of approximately 145 kDa. SHIP-2 is expressed in various tissues including heart brain skeletal muscle and lung. SHIP-2 influences cell growth survival and motility through its enzymatic activity that constrains the levels of PIP3.
Biological function summary
SHIP-2 plays a role in modulating cellular processes like insulin signaling and endocytosis. It acts as a negative regulator counteracting PIP3 accumulation which affects downstream signaling pathways such as Akt. SHIP-2 also integrates into larger protein complexes that contribute to its regulatory functions. These complexes facilitate interactions with multiple cell surface receptors and adaptors emphasizing its role in fine-tuning cell responses.
Pathways
SHIP-2 is an essential component in the insulin signaling and phosphoinositide pathways. It interacts closely with other proteins such as PI3K (phosphoinositide 3-kinase) and PTEN (phosphatase and tensin homolog). In the insulin signaling pathway SHIP-2 modulates the availability of PIP3 impacting the activity of Akt and by extension influence metabolic processes. In the phosphoinositide pathway it harmonizes with PTEN to maintain cellular homeostasis and signaling balance.
SHIP-2 is linked to metabolic diseases like Type 2 Diabetes and obesity. Its regulatory function in insulin signaling positions it as an important player in insulin resistance. Aberrations in SHIP-2 activity can lead to disrupted glucose homeostasis and contribute to the development of these conditions. In the case of insulin signaling disorders SHIP-2's interaction with proteins like IRS-1 (insulin receptor substrate 1) highlights its importance in managing cellular sensitivity to insulin emphasizing its therapeutic potential in metabolic diseases.