Abcam, ab301040, Human LIMK1 knockout A549 cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
LIMK1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout,
Disease:Carcinoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LIMK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LIM Kinase 1 also known as LIMK1 is a serine/threonine protein kinase that plays a critical role in regulating actin cytoskeleton dynamics. Weighing approximately 72 kDa LIMK1 is expressed in various tissues with significant levels observed in the brain and spinal cord. Some studies highlight its elevated presence in neurons. The LIMK1 gene encodes the protein and its activity is often regulated through phosphorylation processes. Researchers often refer to related proteins like LIMK1/proteina in discussions about similar functions.
Biological function summary
This kinase modulates cell shape migration and differentiation by influencing actin filament networks. LIMK1 associates with regulatory complexes and interacts with several proteins to facilitate cytoskeletal reorganization. It plays an essential part in neural development and synaptic plasticity affecting learning and memory. By phosphorylating key actin-binding proteins like cofilin LIMK1 alters actin dynamics essential for various cellular functions.
Pathways
LIM Kinase 1 influences the RhoA/ROCK and Rac1/Pak pathways both critical for cytoskeletal rearrangements. Within these pathways LIMK1 interacts closely with proteins such as cofilin and is influenced by upstream activators like Rho GTPases. These pathways link it to a broader network of signaling that controls cell movement and stability important for cellular responses in various environmental contexts.
LIMK1 is linked to neurological conditions like Williams-Beuren syndrome and some forms of intellectual disability. Changes in LIMK1 expression or activity can disrupt normal brain function leading to cognitive deficits. Its connection to cofilin and other cytoskeleton-associated proteins plays a role in the pathological development of these conditions underlining its importance in maintaining normal neuronal architecture and connectivity.