Abcam, ab301175, Human PPM1D knockout A549 cell line

Size: 1000000Cells / vial
PPM1D KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Disease:Carcinoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control
: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPM1D, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PPM1D protein also known as wild-type p53-induced phosphatase 1 (WIP1) is a serine/threonine phosphatase. Its molecular weight is approximately 66 kDa. PPM1D is an enzyme expressed in various tissues including the brain heart and kidney. This phosphatase plays an important role in the regulation of the cellular stress response by dephosphorylating specific substrates.
Biological function summary
PPM1D/WIP1 acts as a modulator of cellular stress and survival mechanisms. The protein influences the DNA damage response and supports cell cycle progression by deactivating critical checkpoint proteins such as p38 MAPK and p53. It does not form part of a larger protein complex but interacts directly with these proteins to exert its biological functions.
Pathways
PPM1D engages with mechanisms linked to the DNA damage response and the p53 signaling pathway. It counteracts the activation of p53 a protein integral in cell cycle arrest and apoptosis which helps cells to recover post-DNA damage. PPM1D also interacts with proteins like ATM and CHK2 which are part of the DNA repair pathway playing a role in cellular recovery and survival.
PPM1D links to cancer development and progression. Its role in deactivating p53 and facilitating continued cell proliferation makes it relevant to tumorigenesis. Also PPM1D's connection to ATM and the DNA damage response becomes significant in neurodegenerative diseases. Dysregulation of PPM1D can contribute to an improper cellular response to genomic instability contributing to disease pathogenesis.