Abcam, ab302546, Anti-Rab1A antibody [EPR27169-83] (BSA and Azide free)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RAB1A antibody. Carrier free. Suitable for WB, Dot, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27169-83,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:Dot, WB, Flow Cyt (Intra), IP, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rab1A also known as Ras-related protein Rab-1A is a small GTPase belonging to the Rab family. Rab1A has a role in regulating vesicle trafficking and it is involved in ER-to-Golgi transport. This protein with a molecular mass of approximately 23.5 kDa regulates intracellular traffic affecting vesicle budding motility and fusion. Rab1A is highly expressed in various tissues including the liver heart kidney and to lesser extents in others.
Biological function summary
The Rab1A protein influences intracellular transport processes and maintains cellular homeostasis. It forms part of multimeric protein complexes that facilitate its functions in the secretory pathway. The protein ensures the proper trafficking of cargo proteins from the endoplasmic reticulum to the Golgi apparatus playing a role in the early secretory pathway. This involvement is critical for maintaining the post-translational modification and processing of proteins.
Pathways
The mechanical function of Rab1A integrates into several cellular pathways including the secretory pathway and autophagy. Rab1A works with other proteins like Rab2 and Rab30 in the secretory pathway to coordinate the proper flow of proteins. Its coordination with proteins like LC3 during autophagy highlights its importance in cellular turnover and degradation processes linking Rab1A to essential biological functions and homeostasis.
Rab1A has a connection to certain cancers and neurodegenerative diseases. Aberrant Rab1A expression or mutations can lead to disruptions in cellular trafficking implicated in hepatocellular carcinoma. The protein's altered function could also influence the pathogenesis of Parkinson’s disease with proteins such as α-synuclein showing interaction in disease pathways. Understanding these relationships helps in elucidating Rab1A's role in disease mechanisms.