Abcam, ab302617, Anti-cGAS antibody [EPR26492-84]

Size: 20µL / 100µL / 1mL
Anti-cGAS antibody [EPR26492-84] (ab302617) is a rabbit monoclonal antibody detecting cGAS in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26492-84,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-cGAS antibody [EPR26492-84] (ab302617) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of cGAS?
Anti-cGAS [EPR26492-84] (ab302617) specifically detects a band for cGAS (UniProt: Q8N884) at a molecular weight of 59kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR26492-84] also available for your convenience: ab302617, Carrier free -
ab302618
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The cyclic GMP-AMP synthase commonly referred to as cGAS functions as an enzyme that detects cytosolic DNA. It has a molecular weight of 50 kDa. This protein is widely expressed in various cell types with high expression in cells exposed to foreign DNA such as immune cells. cGAS acts by binding to cytosolic DNA which leads to the production of the secondary messenger cyclic GMP-AMP (cGAMP). This action is critical for initiating innate immune responses against viral infections and cellular stress.
Biological function summary
CGAS plays a fundamental role in the immune response by activating the STING pathway. cGAS when bound to DNA forms a complex with DNA as part of its mechanistic function which then catalyzes the synthesis of cGAMP. The cGAMP produced acts as a signaling molecule that activates the STING protein leading to subsequent immune signaling cascades. This activation results in the production of type I interferons and other inflammatory cytokines essential for mounting an effective antiviral response.
Pathways
CGAS operates within important signaling pathways such as the cGAS-STING pathway and the type I interferon pathway. This protein works closely with STING located on the endoplasmic reticulum as a direct effector. The connection between cGAS and STING is important in detecting and responding to cytosolic DNA and initiating immune signaling leading to activation of downstream transcription factors like IRF3 and NF-kB which promote gene expression driving immune responses.
CGAS links to autoimmune diseases and cancer. Aberrant cGAS activity can lead to inappropriate immune activation contributing to autoimmune disorders such as lupus where the body attacks its own cells. In the case of cancer cGAS can play dual roles either promoting an immune response against tumors or contributing to an environment where cancer can evade immune detection. The relation between cGAS and diseases also involves STING as disruptions in this pathway can lead to alterations in immune surveillance and inflammation.