Abcam, ab302788, Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal N-ethylmaleimide-sensitive fusion protein antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25190-1,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N-ethylmaleimide-sensitive fusion protein (NSF) is an important component in the cellular machinery. It unwinds homo-oligomer complexes enabling membrane fusion in cells. NSF also goes by the name of NEM-sensitive factor. It has a mass of about 82 kDa. It is expressed in most tissues where membrane fusion processes occur such as neurons liver and other secretory cells.
Biological function summary
NSF plays a central role in ensuring proper vesicular transport. It is part of the SNARE complex which facilitates the fusion of vesicles with their target membranes. Interaction with small regulatory proteins like SNAPs (Soluble NSF Attachment Proteins) ensures NSF activity in recycling SNARE proteins after vesicle fusion. This process is critical for neurotransmitter release in neuronal synapses and for enzymatic secretion in glandular tissues.
Pathways
NSF contributes significantly to intracellular vesicle trafficking and neurotransmitter release pathways. In these pathways proteins like VAMP synaptobrevin and SNAP-25 interact with NSF to maintain the continuity of vesicle-mediated transport. NSF activity modulates SNARE-mediated vesicle fusion thereby allowing efficient cargo transfer between cellular compartments.
NSF has connections to neurological disorders and certain protein trafficking diseases. Mutations in NSF or dysfunction of its SNARE partners can link to neurodegenerative diseases like Alzheimer’s disease. Proteins like the amyloid-beta precursor protein can also interact aberrantly with NSF pathway components exacerbating disease states. Additionally improper NSF function may relate to cystic fibrosis due to issues in vesicular transport affecting CFTR protein localization.