Abcam, ab302915, Anti-Sp8 antibody [EPR26224-86]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal SP8 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26224-86,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat,
Applications:IHC-Fr, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sp8 also known as Specificity protein 8 is a zinc finger transcription factor. It has a molecular weight of approximately 52 kDa. This protein is expressed primarily in developing tissues including the nervous system and limb buds. It plays an important role during embryonic development by modulating the expression of multiple target genes important for tissue differentiation.
Biological function summary
Sp8 is involved in the regulation of several developmental processes. It functions as a transcription factor that can activate or repress gene expression influencing cell fate during embryogenesis. Sp8 interacts with other transcription factors and cofactors to form larger transcriptional complexes. This protein is also important for the development of the telencephalon and limbs playing a pivotal role in ensuring proper morphological development.
Pathways
Sp8 is an active participant in the Wnt signaling pathway where it contributes to the regulation of gene transcription required for development. In this pathway Sp8 interacts with other factors like β-catenin to promote transcriptional activation of target genes. Sp8 also has connections to the Sonic Hedgehog (Shh) signaling pathway where it influences cell proliferation and differentiation. Both pathways are essential for embryonic development and organogenesis.
Sp8 mutations or dysregulation have been associated with intellectual disabilities and limb malformations. These conditions highlight Sp8’s role in normal neurological and skeletal development. Within these disease contexts Sp8 interacts with proteins such as GLI3 which also plays a role in the developmental abnormalities seen in some syndromes. Understanding the role of Sp8 can provide insights into the molecular mechanisms underlying these disorders.