Abcam, ab303658, Anti-Nav1.5/SCN5A antibody [N397/19] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal SCN5A antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:N397/19,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab303658 is a carrier free version of
ab303657
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nav1.5 also known as SCN5A is a protein that functions mechanically as a voltage-gated sodium channel. This channel facilitates the rapid influx of sodium ions across the cell membrane leading to the depolarization necessary for action potential initiation and propagation in cardiac cells. Nav1.5 weighs approximately 220-260 kDa and is primarily expressed in cardiac myocytes where it plays a central role in maintaining cardiac excitability and conduction.
Biological function summary
The sodium channel Nav1.5 is integral to the electrical signaling within the heart. It functions within the cardiac cell membrane and forms part of the larger sodium channel complex. Nav1.5 maintains ionic balance and supports normal cardiac rhythm making it essential for the contraction of the heart. Its expression and function can be influenced by interacting proteins and intracellular signaling molecules.
Pathways
Nav1.5 is an important component in the cardiac action potential generation and conduction pathways. It directly influences the cardiac conduction system and is part of the pathway that encompasses the sodium-potassium pump and other ion channels like Nav1.1 and Nav1.8. Through these pathways Nav1.5 regulates cardiac rhythm and myocardial contractility ensuring efficient heart function.
Nav1.5 mutations have significant associations with arrhythmias and cardiac conduction issues such as long QT syndrome and Brugada syndrome. These cardiac conditions are linked to disrupted sodium channel function caused by mutations in the SCN5A gene. Nav1.1 and Nav1.2 other family members of the sodium channel can sometimes show altered expression in these disorders reflecting the interconnected nature of this protein family in cardiac health.