Abcam, ab30394, Anti-Integrin beta 1 antibody [12G10]

Size: 100µg
Anti-Integrin beta 1 antibody [12G10] (ab30394) is a mouse monoclonal antibody detecting Integrin beta 1 in  Flow Cytometry, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Over 100 publications - Trusted since 2006
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:12G10,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:The 12G10 epitope has been identified to be within the von Willebrand factor type A domain of the ß-subunit (PubMed ID: 15632175).

Product details:
Product Specifications
Anti-Integrin beta 1 antibody [12G10] (ab30394) is a mouse monoclonal antibody and is validated for use in Flow Cyt and ICC/IF in human samples.
Anti-Integrin beta 1 antibody [12G10] (ab30394) specifically detects Integrin beta 1 (UniProt ID: P05556; Molecular weight: 86kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Integrin beta 1 antibody [12G10] (ab30394) has high sensitivity and specificity.
The specificity of Anti-Integrin beta 1 antibody [12G10] (ab30394) has been confirmed by testing in knockout samples.
Anti-Integrin beta 1 antibody [12G10] (ab30394) has been cited over 109 times in peer reviewed journals and is trusted by the scientific community.
Anti-Integrin beta 1 antibody [12G10] (ab30394) has 7 independent reviews from customers.
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This monoclonal antibody to integrin beta 1 has been knockout validated in ICC/IF and flow cytometry. The expected signal was observed in wild type cells and was not seen in knockout cells.
The 12G10 monoclonal has been shown to increase ligand binding and enhances α5β1 integrin-fibronectin interactions (PubMed ID: 7537221). The antibody promotes cell attachment and spreading when binding to α5β1 integrin but inhibits these processes when binding to α4β1 integrin (PubMed ID: 15632175).
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from tissue culture supernatant., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Integrin beta 1 also known as CD29 ITGB1 or integrin β1 is a part of the integrin family of cell surface receptors. This protein plays a role in cell adhesion and signal transduction processes. Integrin beta 1 weighs around 88 kDa and typically expresses on the surface of various cell types including epithelial endothelial and certain blood cells. As a transmembrane receptor it interacts with extracellular matrix components such as fibronectin collagen and laminin facilitating cell-matrix adhesion which is key in maintaining cellular structure and function.
Biological function summary
Integrin beta 1 functions in cells as a component of heterodimeric complexes with alpha integrin subunits forming adhesion receptors that mediate cellular signaling. These integrins are important for processes like cell migration differentiation proliferation and apoptosis. As they interact with various extracellular ligands integrin beta 1 influences numerous cellular responses particularly in tissue remodeling wound healing and embryonic development.
Pathways
Integrin beta 1 plays significant roles in the MAPK/ERK and PI3K/Akt signaling pathways both of which are essential for cellular growth and survival signals. It forms connections with associated proteins including focal adhesion kinase (FAK) and talin which participate in mechanotransduction and signal propagation. These interactions influence cellular responses to environmental changes impacting processes such as tissue repair and immune response modulation.
Integrin beta 1 associates with cancer progression and fibrosis disorders. Abnormal expression or function of integrin beta 1 can promote tumor invasion and metastasis making it a target for cancer research and potential therapeutic interventions. Furthermore in fibrosis integrin beta 1 interacts with transforming growth factor-beta (TGF-β) signaling contributing to excessive extracellular matrix production and scarring. Understanding these relationships helps in developing targeted treatments for conditions where integrin beta 1 plays a critical role.