Abcam, ab305065, Anti-Plzf antibody [EPR24830-8] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Plzf antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24830-8,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Plzf also known as promyelocytic leukemia zinc finger protein is a transcriptional repressor critical for its mechanical role in gene regulation. It has a mass of approximately 75 kDa. PLZF shows presence in various tissues notably in hematopoietic stem cells testis and certain neural tissues. It functions by binding to specific DNA sequences influencing transcriptional activity. This protein is part of the large BTB/POZ zinc finger family and it plays an engaged role in chromatin remodeling.
Biological function summary
Plzf helps regulate cell differentiation proliferation and self-renewal. Plzf works within several complexes to control gene expression in hematopoietic and germline stem cells. Through these activities it maintains stem cell quiescence and properly times cell differentiation. Plzf impacts neural crest cell fate during embryogenesis and participates in spermatogonia renewal. This involvement in various cellular processes makes it an important protein for development and stem cell biology.
Pathways
Plzf plays a role in pathways such as the Notch signaling and retinoic acid receptor pathways. Within these pathways Plzf interacts with proteins like RAR (retinoic acid receptor) and Hes-1. These interactions affect transcriptional responses that balance stem cell maintenance and differentiation. Plzf's presence in these pathways underlines its importance in controlling genetic programs associated with development and cellular growth.
Plzf associates closely with acute promyelocytic leukemia (APL) and spermatogenic failure. In APL a specific translocation involving the PLZF gene and the retinoic acid receptor alpha (RARA) gene leads to an oncogenic fusion protein disrupting normal myeloid differentiation. This disruption contributes directly to leukemia pathogenesis. In spermatogenic failure abnormal expression or mutation of Plzf impacts testicular function leading to infertility. Studies examine these connections to understand potential therapeutic targets and treatment strategies.