Abcam, ab306214, PE Anti-CD16 antibody [SP189] (C-terminal)

Size: 100µL
Rabbit Recombinant Monoclonal CD16a antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP189,
Isotype:IgG,
Conjugation:PE,
Excitation/Emission:Ex: 480;565nm, Em: 578nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
conjugated primary antibody
is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the
Lightning-Link® conjugation technology
, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.
How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from TCS by protein A/G., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CD16a protein also known as Fc gamma receptor IIIa (FcγRIIIa) plays an important role in the immune system. This receptor is about 50-70 kDa in mass and is expressed on the surface of natural killer (NK) cells macrophages and some T cells. CD16a facilitates antibody-dependent cellular cytotoxicity (ADCC) by binding to the Fc region of immunoglobulin G (IgG). This interaction allows effector immune cells to recognize and destroy antibody-coated target cells.
Biological function summary
CD16a is important for immune responses and occurs as part of a receptor complex. It signals downstream effects through its association with the CD3 zeta chain and the Fc epsilon receptor gamma chain which possess immunoreceptor tyrosine-based activation motifs (ITAMs). Upon binding to IgG CD16a triggers a cascade of events leading to the release of cytokines and cytotoxic granules. This action helps in eliminating infected or cancerous cells demonstrating its role in both innate and adaptive immunity.
Pathways
CD16a contributes significantly to the immunological synapse formation and NK cell activation pathway. This receptor cooperates with other proteins such as CD3 and CD19 to modulate the immune response. It also participates in signaling pathways like the PI3K-Akt pathway which regulates cell survival and proliferation. CD16a's interaction with these pathways highlights its importance in immune cell communication and function.
CD16a is linked to autoimmune diseases and certain cancers. In autoimmune disorders like systemic lupus erythematosus (SLE) abnormal CD16a function can contribute to the pathogenesis by altering immune cell targeting. Additionally in cancers such as lymphoma CD16a's interaction with IgG-coated cancer cells can influence the efficacy of monoclonal antibody therapies. The receptor's association with proteins like CD3 and CD19 in these disease contexts highlights its potential as a therapeutic target.