Abcam, ab308329, Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free

Size: 100µL
Rabbit Recombinant Monoclonal Sodium/Potassium ATPase alpha 1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human, Rat, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Protein within Dog ATP1A1.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL114,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat, Drosophila melanogaster,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Protein within Dog ATP1A1. The exact immunogen used to generate this antibody is proprietary information.P50997

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The alpha 1 Sodium Potassium ATPase often called Na+/K+ ATPase or Na K ATPase plays a critical role in cellular processes by maintaining the electrochemical gradients of sodium (Na+) and potassium (K+) across the plasma membrane. Also known as ATP1 or anti sodium this enzyme facilitates the active transport of Na+ and K+ ions against their concentration gradients a vital function for cellular homeostasis. The molecular weight of this enzyme is approximately 112 kDa with expression observed in various tissues including neuronal and muscular tissues indicating its importance in both central and peripheral cellular functions.
Biological function summary
The alpha 1 Sodium Potassium ATPase establishes ionic balance and is a component of the larger Na K ATPase complex which comprises multiple subunits. This complex not only aids in nerve impulse transmission but also supports muscle contraction and intracellular fluid regulation. Through its activity it significantly influences cellular volume and internal osmotic conditions thereby affecting overall tissue functionality.
Pathways
Alpha 1 Sodium Potassium ATPase is an important player in ion transport and cellular signaling pathways. It closely interacts with pathways such as the renin-angiotensin-aldosterone system and is vital for maintaining blood pressure homeostasis. The enzyme also interacts with several proteins including ankyrin and phospholemman which modulate its activity and are integral to various signaling cascades involved in cardiovascular and renal function.
The dysregulation of alpha 1 Sodium Potassium ATPase activity is linked to conditions like hypertension and congestive heart failure. Malfunctions in this enzyme can lead to improper ionic balance contributing to these diseases. The enzyme interacts with other proteins such as aldosterone synthase in hypertension and these interactions are critical in understanding their pathogenic mechanisms. A greater understanding of these associations may guide therapeutic strategies targeting ion transport abnormalities.