Abcam, ab308357, Anti-SARS CoV 2 COVID 19 nsp7 antibody [HL1301]

Size: 100µL
Rabbit Recombinant Monoclonal SARS-CoV-2 (COVID-19) nsp7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1301,
Isotype:IgG,
Carrier free:Yes,
Reacts with:SARS-CoV-2,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nsp7 also known as non-structural protein 7 is an essential component of the coronavirus replication machinery. This protein has a mass of approximately 83 amino acids and is expressed as part of the viral polyprotein after its synthesis in host cells. Nsp7 plays a mechanical role in stabilizing the SARS-CoV-2 replication complex where it acts alongside other non-structural proteins. It is not generated independently but is an integral element within infected host cells facilitating the viral replication process.
Biological function summary
The non-structural protein 7 of SARS-CoV-2 participates in forming a protein complex involving nsp8 and nsp12 to enhance viral RNA polymerase activity. This complex contributes to the processivity of the viral polymerase by extending the template RNA facilitating rapid and efficient viral RNA synthesis. Nsp7 through its interactions within this complex ensures the stability and proper functionality of the polymerase during viral replication. Its biological role is therefore indispensable in the production of viral progeny during infection.
Pathways
The involvement of nsp7 in viral RNA synthesis pathways is significantly linked to the replication and transcription mechanisms of SARS-CoV-2. Nsp7 in tandem with nsp8 modulates the activity of the RNA-dependent RNA polymerase (nsp12) important for the viral life cycle. This pathway directly impacts the replication fidelity and efficiency of SARS-CoV-2 ensuring successful propagation within the host. Through these interactions nsp7 not only stabilizes but also optimizes the function of the replication-transcription complex.
The activity of nsp7 is fundamental in the context of COVID-19 the disease caused by SARS-CoV-2 infection. The protein orchestrates parts of the viral replication process making it an indirect contributor to COVID-19 pathogenesis. Targeting the replication complex formed by nsp7 nsp8 and nsp12 can provide therapeutic strategies to combat COVID-19. The presence of nsp7 within the replication machinery implicates it in disease progression linking it to respiratory syndrome illnesses associated with SARS-CoV-2.