Abcam, ab308367, Anti-SARS CoV 2 COVID 19 nsp12 antibody [HL1415]

Size: 100µL
Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1415,
Isotype:IgG,
Carrier free:Yes,
Reacts with:SARS-CoV-2,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nsp12 also known as nonstructural protein 12 functions as an RNA-dependent RNA polymerase (RdRp) important for viral RNA genome replication. This protein weighs approximately 106 kilodaltons and derives from the SARS-CoV-2 pathogen the virus responsible for COVID-19. Nsp12 integral to the replication complex is expressed primarily in the host cell's cytoplasm during coronavirus infection. Together with other nonstructural proteins it directs the replication and transcription of the viral genome thereby playing an important role in the viral life cycle.
Biological function summary
Nonstructural protein 12 associates with cofactors nsp7 and nsp8 to form a functional polymerase complex essential for replicating the viral RNA genome. This complex efficiently extends RNA strands from RNA templates using the 3' end as a starting point. Nsp12 contains a conserved polymerase domain which ensures the fidelity of replication. It performs extensive proofreading alongside overlapping proteins like nsp14 which provide exonuclease activity to correct errors sustaining the propagation of the virus within the host.
Pathways
The nsp12 protein directly interacts with mechanisms like viral RNA replication and viral mRNA synthesis. It holds a central position in the coronavirus replication-transcription complex working closely with other nonstructural proteins such as nsp7 and nsp8. These interactions enable the synthesis of full-length genomic RNA and shorter subgenomic RNAs critical for translating viral structural proteins therefore facilitating the continuous cycle of infection and propagation.
Nsp12's activity directly relates to COVID-19 pathogenesis making it a significant target for antiviral treatments. The inhibition of nsp12 through therapeutic agents can effectively disrupt viral RNA synthesis curbing the replication of SARS-CoV-2. Remdesivir a nucleotide analog drug that targets the RdRp activity of nsp12 exemplifies this strategy specifically impeding the SARS-CoV-2 replication process. Additionally variations in nsp12 and its associated protein interactions influence the severity and progression of coronavirus infections in affected individuals.