Abcam, ab309141, Anti-IgE antibody [EPR26777-14]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal IgE antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26777-14,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The IgE protein [also known as immunoglobulin E] is a type of antibody with a molecular mass of approximately 190 kDa. IgE is expressed primarily on the surface of mast cells and basophils. It plays a central role in the immune system by binding to allergens and triggering the release of inflammatory mediators from mast cells and basophils. IgE's high-affinity binding to its receptor FcεRI on these effector cells facilitates this process. IgE's activity is central to initiating immediate hypersensitivity reactions which are a part of the body's immune response.
Biological function summary
The IgE protein functions as a mediator in allergic reactions. It does not operate as part of a complex but directly interacts with allergens and the IgE-specific receptors on immune cells. IgE's role is important in defense against parasitic infections where it performs a protective function by promoting eosinophil activation and degranulation. This action helps the body to expel the parasites. In the absence of parasitic infection IgE's function translates into its role in allergic responses where it becomes responsible for the symptoms associated with allergic diseases.
Pathways
IgE is an important player in the immune response pathway. The protein interacts with allergy-related pathways such as the T helper 2 (Th2) pathway. IgE's role in this pathway involves cooperation with cytokines notably IL-4 and IL-13 which are produced by Th2 cells and promote IgE synthesis in B cells. Also IgE is linked to the FcεRI receptor signaling pathway that initiates the release of histamines and other mediators when IgE binds to an allergen leading to an allergic response.
IgE is closely linked to allergic conditions like asthma and allergic rhinitis. These conditions result from IgE-mediated inflammatory responses that affect the respiratory system. In asthma IgE antibodies play a role in airway inflammation and hyperreactivity by promoting the activation of multiple immune cells including eosinophils and mast cells. IgE's involvement in allergic rhinitis manifests through similar mechanisms with nasal inflammation and irritation. IgE's interaction with other proteins such as histamine-releasing factor exacerbates these conditions by increasing the release of histamine which intensifies the allergic symptoms.