Abcam, ab309310, Human Fibrinogen gamma chain ELISA Kit - Extracellular

Size: 1 x 96Tests
Human Fibrinogen gamma chain ELISA Kit - Extracellular is a Sandwich (quantitative) ELISA for the measurement of Human Fibrinogen gamma chain - Extracellular in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.7 pg/mL,
Range:35.938 - 2300 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Fibrinogen gamma chain - Extracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibrinogen gamma chain - Extracellular protein in human serum, plasma and cell culture supernatant. Quantitate Human Fibrinogen gamma chain - Extracellular with 8.7 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA
REACTIVITY
2,300 pg/mL of recombinant Human Fibrinogen alpha was tested for cross reactivity. No cross reactivity was observed.
2,300 pg/mL of recombinant Human Fibrinogen beta was tested for cross reactivity. <3% cross reactivity was observed.
INTERFERENCE
575 pg/mL of recombinant Human Fibrinogen alpha and 575 pg/mL of recombinant Human Fibrinogen beta was tested for interference with 575 pg/mL of recombinant Human Fibrinogen gamma. No interference was observed. 575 pg/mL of recombinant Human Fibrinogen beta increased interpolated value of 575 pg/mL of recombinant Human Fibrinogen gamma 5.7%.
SPECIES REACTIVITY
Other species reactivity was determined by measuring 1:1000 serum samples of various species, interpolating the protein concentrations from the species standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in species serum assayed at the same dilution.
Reactivity of 77% was determined for primate serum.
No signal was observed in 1:1000 serum samples from the following species: Mouse, Rat, Bovine
Other species reactivity not determined.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C