Abcam, ab309314, Human Periostin ELISA Kit

Size: 1 x 96Tests
Human Periostin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Periostin with a sensitivity of 20.98 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Sample types:Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Cell culture media,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 20.98 pg/mL,
Range:78.125 - 5000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Periostin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Periostin protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Periostin with 20.98 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.
Biological function summary
The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.
Pathways
Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.
The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.