Abcam, ab309967, Anti-HCoV-OC43 Nucleoprotein antibody [abd385] - BSA and Azide free

Size: 100µg / 1mg
Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Human coronavirus OC43, Recombinant fragment - Human coronavirus OC43 samples.
Key facts
Host species:Human,
Clonality:Monoclonal,
Clone number:abd385,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Human coronavirus OC43,
Applications:I-ELISA, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody does not cross-react with recombinant HCoV-NL63 nucleocapsid, MERS-CoV nucleocapsid, SARS-CoV nucleocapsid, HKU1 nucleocapsid and HCoV-229E nucleocapsid.

Product details:
ab309967 is the carrier-free version of
ab309966
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HCoV-OC43 Nucleoprotein also known as OC43 N protein is a major structural component of the HCoV-OC43 coronavirus. This nucleoprotein has a mass of approximately 50 kDa. It is expressed within the infected host cells mainly in the cytoplasm where it plays an integral role in viral replication and assembly. The HCoV-OC43 Nucleoprotein ensures encapsidation of the viral RNA genome forming the ribonucleocapsid which is critical for protecting the viral RNA and aiding in its functionality within the cell.
Biological function summary
The HCoV-OC43 Nucleoprotein participates in forming complexes with viral RNA and other viral proteins. These interactions facilitate the replication cycle of HCoV-OC43 by regulating RNA transcription and synthesis. Additionally it is known to induce host responses influencing cellular processes and immune evasion mechanisms. This interaction with the host's cellular machinery enables the virus to establish infection and maintain its replication efficiency.
Pathways
HCoV-OC43 Nucleoprotein participates in essential viral life cycle pathways specifically in the replication and transcription complex formation. This protein associates with other viral entities like the RNA-dependent RNA polymerase and matrix protein M. Together they ensure the synthesis of subgenomic mRNA and full-length genomic RNAn important for the successful production of new virions. Understanding these pathways helps clarify how coronaviruses like HCoV-OC43 propagate and sustain infection within host cells.
HCoV-OC43 Nucleoprotein associates with respiratory infections including common colds and occasionally severe lower respiratory tract diseases. It plays a role in modulating host immune responses that contribute to disease severity. It often connects with host protein interactions such as those involving the immune modulator protein interferon which can either bolster or subvert immune defenses. Studies of HCoV-OC43 and its Nucleoprotein provide insight into coronavirus pathogenicity and potential therapeutic targets for viral respiratory illnesses.