Abcam, ab310912, PE Anti-ENT1 antibody [SP120]

Size: 100µL
Rabbit Recombinant Monoclonal ENT1 antibody - conjugated to PE.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP120,
Isotype:IgG,
Conjugation:PE,
Excitation/Emission:Ex: 480;565nm, Em: 578nm,
Carrier free:No,
Applications:Antibody Labelling, Target Binding AffinitySee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ENT1 also known as Equilibrative Nucleoside Transporter 1 or SLC29A1 is a membrane protein responsible for transporting nucleosides across cell membranes. This protein weighs approximately 55 kDa and is expressed in various tissues including the brain heart and liver. The transport activity of ENT1 plays a fundamental role in maintaining cellular nucleoside levels. The efficient transport mechanisms of ENT1 highlight its significance in regulating the availability of substrates for nucleic acid synthesis.
Biological function summary
This transporter facilitates the uptake and recycling of nucleosides which are essential building blocks for DNA and RNA synthesis. ENT1 mediates the transport of adenosine an important nucleoside involved in multiple cellular functions. It operates as a component of a broader transport system that includes similar proteins like ENT2. This action contributes to cellular processes by controlling nucleoside concentrations impacting cell proliferation and metabolic activities.
Pathways
ENT1 plays a role in the purine salvage pathway and adenosine signaling. It regulates extracellular adenosine levels influencing adenosine receptor-mediated pathways that impact many physiological functions. ENT1 coordinates with proteins such as adenosine kinases to manage the intracellular balance of adenosine therefore integrating into both metabolic and signaling routes. Its role in these pathways affects energy metabolism and supports cellular responses to stress.
ENT1 has been associated with conditions such as cardiovascular diseases and neurological disorders. Altered ENT1 activity can affect adenosine levels leading to variations in blood flow and neurotransmission. In cardiovascular diseases ENT1 modulates adenosine's cardioprotective effects. In neurological contexts dysfunction of ENT1 relates to disorders involving adenosine receptor signaling where its interaction with proteins like ADA (adenosine deaminase) can influence disease progression.