Abcam, ab313374, Anti-DNA Ligase III/LIG3 antibody [EPR27265-44]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal DNA Ligase III/LIG3 antibody. Suitable for Flow Cyt (Intra), IHC-P, IP, ICC/IF, WB and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27265-44,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WB, Flow Cyt (Intra), ICC/IF, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for human IHC-P, and rat ICC and FC-Intra.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DNA Ligase III also known as LIG3 or Lig 3 is an enzyme that plays a central role in DNA repair by catalyzing the joining of DNA strands. Mechanically it facilitates the ligation step in the DNA repair process sealing nicks in the DNA backbone. This ligase has a sizing mass of approximately 103 kDa. It is expressed widely in human cells with a high concentration in tissues with active DNA repair needs such as testes and ovaries. Ligase acts in various subcellular locations including the nucleus and mitochondria with distinct isoforms functioning in these compartments.
Biological function summary
DNA Ligase III is significant in maintaining genomic stability. It often operates as part of multi-protein complexes interacting especially with XRCC1 during base excision repair (BER). It ensures the continuity of DNA strands after damage-induced breaks are processed. LIG3 also has a pivotal function in the mitochondria where it is involved in repair and replication of mitochondrial DNA therefore preserving mitochondrial genome integrity.
Pathways
DNA Ligase III is an integral part of the DNA repair pathways particularly the base excision repair (BER) and single-strand break repair (SSBR) pathways. In the BER pathway LIG3 collaborates closely with other proteins like XRCC1 to mend damaged DNA by filling in missing nucleotides and sealing nicks. It also plays a role in non-homologous end joining (NHEJ) though this is more dominantly accomplished by DNA Ligase IV. These pathways are important for correcting oxidative damage and abasic sites that can disrupt the DNA structure.
DNA Ligase III has associations with certain syndromes and cancers. Mutations or malfunctions in this enzyme can lead to ataxia-telangiectasia-like disorder owing to impaired DNA repair processes. Additionally LIG3 expression is often evaluated in the context of cancerous cells where aberrant repair processes could promote tumor progression. A connection with XRCC1 another key player in the repair machinery is critical as XRCC1 mutations may magnify the impact of LIG3 deficiencies contributing to disease pathology.