Abcam, ab313492, Anti-p95/NBS1 antibody [RP23040230]

Size: 100µg
Anti-Anti antibody [RP23040230] is a Rabbit Recombinant Multiclonal antibody that is used in Anti ICC/IF, Western Blot. Suitable for Human, Rat samples. p95/NBS1 is a component of th eMRN complex which plays an important role in double-strand break repair, DNA recombination, maintenance of telomere integrity and meiosis.The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases of the cell cycle.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:RP23040230,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NBN.O60934

Product details:
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
View our range of
recombinant multiclonal antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target known as p95/NBS1 also referred to as NBN protein is a critical component in the DNA damage response mechanism. The full molecular weight of the NBS1 protein is approximately 95 kDa. This protein is abundantly expressed in various tissues particularly where there is a higher rate of cell division or repair such as in the thymus and testis. It plays an important role in maintaining the stability of the genome through its involvement in the repair of double-strand breaks.
Biological function summary
The NBS1 protein functions as an essential component of the MRN complex which also includes MRE11 and RAD50 proteins. This complex is fundamental in accurately detecting DNA double-strand breaks and initiating repair processes. Through its actions NBS1 facilitates numerous cellular processes that preserve genomic integrity such as homologous recombination and non-homologous end joining. Its influence in controlling the cell cycle further emphasizes its role in maintaining cellular health.
Pathways
The NBS1 protein engages significantly in the DNA damage response and repair pathways notably impacting the ATM signaling pathway. By interacting with proteins such as ATM kinase NBS1 facilitates the phosphorylation and activation of several downstream effectors necessary for DNA repair and cell cycle checkpoints. The MRN complex also links with the RAD50 protein highlighting its involvement in these pathways.
Mutations in the NBS1 gene are linked to Nijmegen breakage syndrome characterized by sensitivity to radiation immune deficiency and increased cancer risk. Additionally NBS1's role in pathways makes it pertinent to certain cancer pathologies where its malfunction can contribute to genomic instability. The NBN protein through its interaction with ATM and RAD50 assists in understanding its connection with predispositions to these diseases.