Abcam, ab313682, Human CFC1 ELISA Kit

Size: 1 x 96Tests
Human CFC1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CFC1 in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 4.3 pg/mL,
Range:4.3 - 1000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CFC1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CFC1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CFC1 coated on a 96-well plate.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CFC1 also known as cryptic family member 1 is a 36 kDa protein that plays a significant role in embryonic development. This protein is located in the extracellular matrix and the cellular surface where it interacts with various cell signaling molecules. It is predominantly expressed in the developing heart during gastrulation and in other embryonic tissues suggesting its importance in early development stages. CFC1 interacts with Nodal signaling pathways acting as a co-factor necessary for proper signal transduction.
Biological function summary
Cryptic family member 1 is essential in vertebrates for cell differentiation processes and can form part of a protein complex. This complex is involved in the regulation of left-right asymmetry during embryogenesis ensuring correct organ positioning and development. CFC1 functions by aiding Nodal protein signaling which affects the development of important structures in the early embryonic phase including the heart and other organ systems.
Pathways
CFC1 engages directly with the Nodal signaling and Hedgehog signaling pathways. These pathways have a significant role in embryonic patterning and organogenesis. Within these pathways CFC1 modulates interactions that directly influence Nodal and Hedgehog proteins. Nodal signaling is important in left-right patterning while Hedgehog plays a role in organ differentiation and positioning. The proper functioning of these pathways ensures the correct spatial organization of organs.
Anomalies in CFC1 are linked to heterotaxy syndrome and congenital heart defects. Disruptions in CFC1 may lead to misalignment in organ positioning due to asymmetrical body axis formation. Nodal protein-related disorders manifest when CFC1's function is impaired leading to disrupted left-right signal transmission and resulting in abnormal organ development and placement. Understanding CFC1's role is critical for grasping how such developmental diseases manifest and may guide future therapeutic interventions.