Abcam, ab313733, Alexa Fluor® 647 Anti-IRF8 antibody [EPR26382-10]

Size: 100µL
Rabbit Recombinant Monoclonal IRF8 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26382-10,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IRF8 also known as Interferon Regulatory Factor 8 plays a significant role as a transcription factor in the regulation of gene expression. With a molecular mass of approximately 48 kDa this nuclear protein primarily functions in immune cells such as monocytes dendritic cells and macrophages. It binds to specific DNA sequences in gene promoters influencing the transcription of genes involved in immune response and development of blood cells. The expression of IRF8 extends beyond immune cells to tissues like the spleen and thymus.
Biological function summary
IRF8 drives differentiation and maturation of various immune cell types. It forms part of protein complexes that control the expression of genes necessary for immune surveillance and defense mechanisms. IRF8 can dimerize with other IRF family members or proteins like PU.1 to modulate activity of specific target genes. This transcription factor supports the growth and differentiation of myeloid cells and helps to balance lymphoid and myeloid lineage commitment which is important for maintaining a proper immune system function.
Pathways
IRF8 is integral to the interferon signaling and hematopoietic cell lineage pathways. It enhances the expression of interferon-stimulated genes integrating signals for a potent antiviral response. The protein connects with transcriptional regulators such as IRF1 and STAT1 to modulate these pathways. By participating in hematopoietic differentiation IRF8 also influences pathways related to the immune system development and inflammatory responses.
Mutations or alterations in IRF8 function associate with immunodeficiencies and hematologic malignancies. Chronic myelogenous leukemia and systemic lupus erythematosus exemplify conditions where IRF8 plays a role. Its dysfunction affects the regulation of cytokine genes which can lead to inappropriate immune responses. The protein's activity intersects with other key players such as BTK and BCL2 which are involved in disease processes related to immune cell proliferation and survival.