Abcam, ab313745, Anti-Influenza A Virus PA protein antibody [EPR28250-39]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal Polymerase acidic protein antibody. Suitable for I-ELISA, WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Recombinant fragment - Influenza A, Transfected cell lysate - Influenza A, Transfected cell line - Influenza A samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28250-39,
Isotype:IgG,
Carrier free:No,
Reacts with:Influenza A,
Applications:ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Influenza A virus PA protein also known simply as PA protein plays an important mechanical role in the viral replication process. It acts as a component of the RNA polymerase complex within the Influenza A virus. This protein has a molecular mass of approximately 85 kDa. PA is expressed in infected host cells and contributes significantly to viral RNA synthesis by possessing endonuclease activity which is important for the cleavage of host mRNA during viral transcription.
Biological function summary
This protein is part of the viral RNA polymerase complex along with PB1 and PB2 proteins. This complex functions as an essential machinery for the transcription and replication of the viral RNA genome enabling the virus to use host cellular machinery for its proliferation. PA protein interacts specifically with the PB1 protein assisting in the polymerase assembly and activity which marks it as an important element in the life cycle of the virus.
Pathways
The PA protein is involved primarily in the influenza viral RNA synthesis pathway. The polymerase complex which includes PA drives this pathway by transcribing viral RNA into mRNA and replicating the viral genome. The PA protein also interacts with the host's nuclear pathways as influenza replication occurs in the host cell nucleus. Its interaction with PB1 and PB2 proteins is central to these pathways facilitating the initiation and elongation phases of RNA synthesis.
The PA protein relates directly to influenza infections in humans which can manifest as seasonal flu outbreaks or pandemic events. Its role in the replication of Influenza A makes it a potential target for antiviral drug development. Compounds that inhibit the endonuclease activity of PA protein aim to impair viral growth and spread. The PA protein's interaction with other viral proteins like PB1 highlights its involvement in the pathogenesis of influenza and highlights its importance in the disease life cycle.