Abcam, ab313795, Anti-MYO5B antibody [EPR28275-55]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal MYO5B antibody. Suitable for Flow Cyt (Intra), ICC/IF, WB, Dot and reacts with Mouse, Rat, Human, Synthetic peptide - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28275-55,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WB, Dot, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for human FC-Intra and ICC/IF.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MYO5B also known as myosin-Vb is a motor protein that plays a significant role in cellular transport. It exhibits a molecular mass of approximately 215 kDa. MYO5B interacts with actin filaments conducting transport of cargo through the cell via ATP hydrolysis. It is chiefly found in epithelial cells such as those lining the intestines and kidneys. This protein localizes to various intracellular compartments including the plasma membrane and endosomal compartments facilitating organelle and protein transport critical for maintaining cellular function.
Biological function summary
The mechanical movements generated by MYO5B are essential for intracellular trafficking processes. It serves as a component of the transport machinery involving membrane trafficking ensuring the proper distribution of cellular materials. As part of a molecular complex MYO5B works in conjunction with Rab GTPases to regulate vesicle movement. This coordination helps maintain the polarity and integrity of epithelial cells which are important for nutrient absorption and secretion in tissues.
Pathways
MYO5B is intimately involved in the regulation of the endocytic recycling pathway. It plays a pivotal role in the recycling of receptors and lipids back to the plasma membrane. The protein interfaces with key elements such as RAB11A which is important for the recycling pathway. MYO5B’s function in this pathway is integral for maintaining epithelial cell dynamics and tissue homeostasis ensuring correct receptor and nutrient transporter localization in the cellular membrane.
Dysfunctional MYO5B is implicated in microvillus inclusion disease (MVID) a disorder that disrupts nutrient absorption in the intestines. This protein forms links with RAB11A and promotes disruptions when mutated leading to the symptoms observed in MVID. Additionally MYO5B mutations have associations with cholestasis a condition where bile cannot flow from the liver to the duodenum. The relationship with other proteins like syntaxin 3 further complicates cholestasis pathophysiology highlighting MYO5B’s broad impact in human diseases.