Abcam, ab313862, Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free

Size: 100µL
Rabbit Recombinant Monoclonal KMT1E / SETDB1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SETDB1.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1645,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SETDB1. The exact immunogen used to generate this antibody is proprietary information.Q15047

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KMT1E also known as SETDB1 is a lysine methyltransferase with a role in epigenetic regulation. This enzyme has a molecular weight of approximately 143 kDa. It actively methylates histone H3 at lysine 9 (H3K9) a modification associated with transcriptional repression. SETDB1 contains a SET domain responsible for its methyltransferase activity. Expressed mainly in the nucleus this enzyme plays a critical role in controlling gene expression across various tissues including the brain spleen and testis.
Biological function summary
The methyltransferase SETDB1 impacts chromatin structure and gene silencing. It operates as a part of the histone methylation complex collaborating with co-factors like ATF7IP and MBD1 to achieve highly specific gene repression. By catalyzing trimethylation of H3K9 it creates a binding site for HP1 proteins facilitating the formation of heterochromatin. This action is essential for processes such as X-chromosome inactivation and the repression of transposable elements.
Pathways
Regulation through SETDB1 influences key biological processes. This enzyme is integral to the PRC2 (Polycomb Repressive Complex 2) pathway maintaining transcriptional silencing during development. Furthermore SETDB1 works alongside SUV39H1 in dynamic change of heterochromatin states regulating genes involved in cell cycle and differentiation. Such pathways highlight its significant interactions with proteins like EZH2 and EED which assist in maintaining stable gene silencing.
SETDB1 shows a connection with cancer particularly in melanoma and lung cancer. Overexpression or dysregulation of SETDB1 can lead to aberrant methylation patterns contributing to oncogenesis through alteration of tumor suppressor genes. Additionally schizophrenia has an association with SETDB1 where improper histone modification can impact gene expression programs critical for neurological function. In these contexts proteins such as DNMT1 also play a role as they collaborate in epigenetic modifications linked to the disease development.