Abcam, ab314065, Anti-Lasu1/Ureb1 antibody [BLR129H] - BSA free

Size: 100µL
Rabbit Recombinant Monoclonal HUWE1/Mule antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human HUWE1 aa 2250-2300.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:BLR129H,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:ICC, Flow Cyt, IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human HUWE1 aa 2250-2300. The exact immunogen used to generate this antibody is proprietary information.Q7Z6Z7

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.8 - 8.6Preservative: 0.09% Sodium azideConstituents: 98% Borate buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein HUWE1 also known as Mule Mule HRP or Mule Mouse is a large HECT domain E3 ubiquitin ligase with a molecular weight of approximately 437 kDa. It functions by attaching ubiquitin to substrate proteins marking them for degradation via the proteasome pathway. HUWE1 is expressed ubiquitously in tissues but shows higher expression levels in brain testis and certain cancers. It regulates protein homeostasis highlighting its importance in cellular processes.
Biological function summary
HUWE1/Mule modulates various cellular functions through its ubiquitin ligase activity. It is not a part of a larger complex but interacts with numerous proteins influencing cell cycle regulation apoptosis and DNA repair. Its role in apoptosis involves facilitating the degradation of the pro-apoptotic protein Mcl-1 thereby promoting cell death. Additionally HUWE1 contributes to DNA repair by targeting histone H2AX for modification which is an important step in the DNA damage response.
Pathways
HUWE1 acts within the ubiquitin-proteasome system and the DNA damage response pathway. In the ubiquitin-proteasome system HUWE1 closely interacts with proteins such as p53 and c-Myc playing a significant role in regulating their stability and function. In the DNA damage response pathway the protein's activity affects the signaling required for cell cycle checkpoints underlining its relevance in maintaining genomic stability.
HUWE1 mutations and dysregulation link to intellectual disability and certain cancers. Its association with intellectual disability arises from alterations in neural gene regulation partly through its interaction with proteins like p53. In cancer aberrant activity of HUWE1 results in uncontrolled proliferation as it affects the degradation of critical proteins involved in tumor progression such as c-Myc. Understanding HUWE1's mechanisms can provide insights into these conditions and potential therapeutic strategies.