Abcam, ab314214, Anti-Beta Arrestin 2 antibody [EPR25410-70] - BSA and Azide free

Size: 100µg / 1mg
Knockout Tested Rabbit Recombinant Monoclonal Beta Arrestin 2 antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25410-70,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab314214 is the carrier-free version of
ab314213
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta-arrestin 2 also known as arrb2 or b-arrestin mechanically functions to regulate G protein-coupled receptor (GPCR) signaling. This protein has a molecular mass of approximately 47 kDa and is ubiquitously expressed across various tissues including the brain lungs and heart. Beta-arrestin 2 works by binding to phosphorylated GPCRs facilitating receptor desensitization and endocytosis which stops the receptor's signaling activity and mediates receptor internalization.
Biological function summary
Beta-arrestin 2 plays an important role in mediating signal transduction pathways beyond GPCR desensitization including those involved in cell communication and cellular response regulation. Beta-arrestin 2 interacts with multiple signaling proteins to form complexes that facilitate signaling pathways notably influencing MAPK (Mitogen-Activated Protein Kinase) pathways. It provides a scaffold for downstream signaling molecules affecting cell signaling outcomes.
Pathways
Beta-arrestin 2 significantly participates in two important biological pathways: the MAPK pathway and the Wnt signaling pathway. Through these pathways beta-arrestin 2 interacts with signaling proteins such as ERK1/2 (Extracellular signal-Regulated Kinases) and dishevelled proteins. These interactions highlight beta-arrestin 2's ability to modulate signal strength and duration contributing to diverse cellular responses.
Beta-arrestin 2 is implicated in conditions like heart failure and certain cancers. The aberrant regulation of beta-arrestin 2-related pathways can lead to uncontrolled cell proliferation or inadequate heart responses. Additionally beta-arrestin 2 is connected to proteins like GPCR kinases in the context of heart failure and MAPK proteins within oncogenic processes underlining its potential as a therapeutic target.