Abcam, ab314257, APC Anti-CX3CR1 antibody [EPR22354-15]

Size: 100µL
Rabbit Recombinant Monoclonal CX3CR1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22354-15,
Isotype:IgG,
Conjugation:APC,
Excitation/Emission:Ex: 650nm, Em: 660nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CX3CR1 also known as fractalkine receptor is a seven-transmembrane G protein-coupled receptor. It has a molecular weight of about 40 kDa. This receptor is expressed in various tissues particularly on immune cells like monocytes natural killer cells T cells and microglia. CX3CR1 binds to its ligand CX3CL1 or fractalkine which is a chemokine involved in directing cell movement. The receptor-ligand interaction is critical for the adhesion and migration of leukocytes to inflammatory sites.
Biological function summary
CX3CR1 plays a significant role in immune regulation and cellular migration. It is not part of a larger protein complex but interacts directly with monocyte and microglia cells influencing their activity. CX3CR1 is recognized as a marker for microglia in flow cytometry applications assisting in the identification of these brain-resident immune cells. The receptor is frequently studied using monoclonal antibodies like anti-CX3CR1 and is subject to inhibition by CX3CR1 antagonists such as 8e10 affecting its function in immune responses.
Pathways
CX3CR1 involves itself heavily in the chemokine signaling pathway and the inflammatory response pathway. Its interaction with CX3CL1 influences the PI3K-Akt signaling pathway modulating cell survival and proliferation. The receptor works in conjunction with CCR2 in monocyte navigation impacting the inflammatory cascade during immune response. Their coordinated action is important for cellular trafficking to sites requiring immune intervention.
CX3CR1 has associations with neurodegenerative diseases such as Alzheimer's disease as well as with atherosclerosis. Changes in its expression and function can exacerbate these conditions. In Alzheimer's disease microglial CX3CR1 may mediate neuroinflammatory processes affecting plaque buildup. In atherosclerosis the receptor is involved in monocyte recruitment to plaques influenced by the related protein fractalkine. Understanding these relationships provides insights into potential therapeutic targets for modulating disease progression.