Abcam, ab314380, Human Robo3 ELISA Kit

Size: 1 x 96Tests
Human Robo3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Robo3 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Serum, Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.06 ng/mL,
Range:0.06 - 15 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Robo3 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Robo3 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Robo3 coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen, B7-H1, BMPR-IA, BMPR-II, Cadherin-11, CD27, CD6, Ck beta 8-1, CNTF, DNAM-1, CD147, FLRG, Follistatin-like 1, Fractalkine, Galectin-1, GITR Ligand, Granulysin, IL-1 R3, IL-15 R alpha, IL-17E, IL-32 alpha, CHL-1, LRIG3, LRP-6, MEPE, Nectin-4, Periostin, Persephin, Renin, RGM-B, S100 A8, Siglec-7, Syndecan-3, Thrombospondin 2, Thrombospondin 5, Tie-1, ULBP-2.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Robo3 also known as Roundabout homolog 3 or Rig1 is a receptor protein with a mass of approximately 117 kDa. It is expressed in the developing nervous system especially in commissural neurons of the spinal cord. Mechanically Robo3 plays a major role in axon guidance. It does not bind to Slit proteins like other Robo family members but it inhibits Slit-induced repulsion allowing axons to cross the midline.
Biological function summary
The protein facilitates proper nerve cell navigation. It is essential for enabling axons to reach their correct targets by modulating guidance cues. Robo3 is not known as part of a complex but interacts with other guidance signals to steer commissural axons during neural development. Without its functional expression axons fail to cross the midline leading to improper neural circuit formation.
Pathways
Robo3 interacts with signals central to neural circuit development. It mediates important guidance pathways such as the Netrin-DCC pathway working alongside Netrin-1 to promote axonal growth. Robo3 functions in a pathway with other guidance proteins like Robo1 and DCC to ensure neuronal pathfinding is achieved coordinating the switch from attraction to repulsion after midline crossing.
Robo3's malfunction is linked to horizontal gaze palsy with progressive scoliosis (HGPPS) a rare genetic condition affecting eye movements and spinal curvature. Misregulation of related proteins like L1CAM exacerbates navigational errors in axon guidance contributing to the disorder's characteristics. These insights underline the significance of Robo3 in maintaining normal neurological function and its implications in neurodevelopmental conditions.