Abcam, ab314381, Human RSPO2 ELISA Kit

Size: 1 x 96Tests
Human RSPO2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human RSPO2 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Serum, Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.55 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human RSPO2 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human RSPO2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human RSPO2 coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM22, Arylsulfatase B/ARSB, B3GNT2, Carbonic Anhydrase VB, Caspase 7, Caspase 8, Integrin alpha M/CD11b, SIRP gamma/CD172g, CD39L2/ENTPD6, CD39L4/ENTPD5, Integrin alpha 2/CD49b, CD7, CEACAM-3, Carboxypeptidase E/CPE, FABP6, FAM3C, GDF-3, GSTM1, Kallikrein 11, Kallikrein 12, Kremen-2, OSCAR, PTP1B, Reg3A, S100A13, Semaphorin 4C, Sirtuin 1/SIRT1, SMPD1, Sortilin, SPINK1, Stabilin-2, SULT2A1, Transcobalamin II, THSD1, TrkA, UCH-L3, VAPA, vWF-A2, Wnt-4.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RSPO2 also known as R-spondin-2 is a secreted protein weighing approximately 35 kDa. It acts mechanically by potentiating the Wnt signaling pathway. This protein is part of the R-spondin family and contains a signal peptide followed by two furin-like repeat domains a thrombospondin type 1 domain and a C-terminal region. RSPO2 is expressed in various tissues with notable expression in the lung muscle and reproductive organs.
Biological function summary
The protein enhances Wnt signaling which is important for the regulation of cellular processes including proliferation and differentiation. RSPO2 interacts with LGR receptors and is essential in modulating tissue development and homeostasis. The protein forms part of a complex with ZNRF3 triggering removal of negative regulators from the cell surface. This fine regulation allows RSPO2 to play roles in skeletal and organ development.
Pathways
RSPO2 actively participates in the canonical Wnt/β-catenin signaling and non-canonical pathways. Within the Wnt/β-catenin pathway RSPO2 collaborates with LRP6 and Frizzled receptors to stabilize β-catenin which then translocates to the nucleus to influence gene expression. In pathways regulating tissue homeostasis RSPO2 works with RNF43 to control ZNRF3 activity affecting how many receptors are available for Wnt signaling.
RSPO2 shows involvement with craniofacial development disorders and certain cancers. Mutations or dysregulation of RSPO2 and its partners such as LRP6 can lead to craniofacial deformities stemming from improper cell signaling during embryonic development. In cancer RSPO2 may contribute to tumor growth due to its role in enhancing Wnt signaling which is often activated aberrantly in tumor cells. The interplay with ZNRF3 in cancer illustrates its wider impact on tissue overgrowth and malignant transformation.