Abcam, ab314432, Anti-pan Brd4 antibody [EPR25424-71]

Size: 20µL / 100µL / 1mL
Anti-pan Brd4 antibody [EPR25424-71] (ab314432) is a rabbit monoclonal antibody detecting pan Brd4 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ChIP . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25424-71,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:ChIP-seq, WB, IHC-P, IP, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-pan Brd4 antibody [EPR25424-71] (ab314432) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human, Mouse, Rat samples.
What is the molecular weight of pan Brd4?
Anti-pan Brd4 [EPR25424-71] (ab314432) specifically detects a band for pan Brd4 (UniProt: O60885) at a molecular weight of 152kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Other related products
We have a range of other formats of antibody clone [EPR25424-71] also available for your convenience: ab314432, Carrier free -
ab314433
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pan Brd4 also known as bromodomain-containing protein 4 belongs to the BET family. This protein often acts as an epigenetic reader recognizing acetylated lysine residues on histone tails. Brd4 has two bromodomains which contribute to its function in regulating gene expression by facilitating the assembly of transcriptional complexes. The Brd4 molecular weight varies depending on its isoforms with the short form around 75 kDa and the long form approximately 150 kDa. Brd4 is expressed widely across various tissues including the heart brain and testis.
Biological function summary
Brd4 plays an essential role in transcriptional regulation and control of gene expression. It interacts with multiple transcription factors and serves as a critical component of the super-enhancer complex where it aids in the recruitment of RNA polymerase II. Brd4 links the chromatin environment to transcription machinery facilitating transitions from chromatin condensation to active transcription. Furthermore Brd4 involvement extends to the regulation of cell cycle progression and mitotic bookmarking ensuring the retention of transcriptional memory through cell divisions.
Pathways
Brd4 integrates into vital cellular processes influencing inflammation and cancer pathways. In the context of the inflammatory pathway Brd4 associates with NF-kB a significant transcription factor in immune response regulation. For cancer pathways Brd4 influences MYC a well-recognized oncogene by driving its expression and subsequent cellular proliferation. These interactions highlight Brd4 as a prominent node in transcription regulation connecting to significant proteins involved in cellular growth and inflammation responses.
Brd4 significance emerges in cancer and cardiovascular diseases. In cancer such as acute myeloid leukemia Brd4 aberrantly regulates MYC resulting in uncontrolled cell proliferation and tumor growth. The connection here with MYC underpins Brd4's pivotal role in oncogenesis. In cardiovascular conditions Brd4 influences inflammatory gene expression contributing to pathologies like atherosclerosis. These roles make Brd4 a target for therapeutic development with inhibitors being investigated for their potential in treating cancers and inflammatory disorders associated with this protein.