Abcam, ab314741, Anti-Rfx Cas13d (N-term) antibody [EPR23965-31]

Size: 20µL / 100µL / 1mL
Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741) is a rabbit monoclonal antibody detecting Rfx Cas13d (N-term) in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Ruminococcus flavefaciens . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23965-31,
Isotype:IgG,
Carrier free:No,
Reacts with:Ruminococcus flavefaciens,
Applications:ICC/IF, IHC-P, IP, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (ab314741) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Ruminococcus flavefaciens samples.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Other related products
We have a range of other formats of antibody clone [EPR23965-31] also available for your convenience: ab314741, Carrier free -
ab314742
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rfx Cas13d also known as C2c10 functions as a versatile RNA-targeted CRISPR-associated protein. Its primary mechanical action involves RNA-guided RNase activity facilitated by a guide RNA that directs the cleavage of specific RNA targets. This protein weighs approximately 930 amino acids and is expressed in various prokaryotic organisms. Engineered versions of Cas13d like Rfx Cas13d have been adapted for use in eukaryotic cells enabling targeted gene silencing and manipulation of RNA transcripts.
Biological function summary
Rfx Cas13d contributes to RNA processing and regulation playing a role in RNA interference mechanisms. It associates with RNA molecules and degrades them therefore regulating gene expression post-transcriptionally. Rfx Cas13d does not act alone; it forms part of a complex with guide RNA that determines its specificity. Its ability to cleave RNA makes it valuable for studying RNA function and interactions within cells offering insights into RNA-based regulation processes.
Pathways
Rfx Cas13d influences specific RNA regulatory pathways that control gene silencing and expression. It participates in pathways associated with RNA interference and endogenous processes similar to microRNA (miRNA) pathways. In these pathways it relates to proteins like AGO (Argonaute) which also play roles in RNA-induced silencing complexes. Through its RNA cleavage activity it helps manage RNA populations within cells ensuring correct gene expression regulation.
Rfx Cas13d holds potential for therapeutic applications especially in targeting RNA viruses and genetic diseases caused by RNA dysregulation. For example its ability to degrade viral RNA makes it a prospective tool in combating viral infections such as influenza. Also it may relate to disorders involving misregulated RNA species which in some cases connect to malfunctioning proteins thereby providing potential intervention points. Cas13d's action could help counteract diseases by restoring normal RNA regulation when linked to defective proteins or pathways.