Abcam, ab314749, Anti-USP30 antibody [EPR27024-81]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal USP30 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27024-81,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP30 also known as ubiquitin specific peptidase 30 functions as a deubiquitinating enzyme. It has a mass of about 59 kilodaltons. This enzyme removes ubiquitin from protein substrates playing an important role in the regulation of protein degradation. USP30 is normally expressed in mitochondria where it participates in maintaining protein homeostasis by removing polyubiquitin chains.
Biological function summary
The deubiquitinating activity of USP30 helps maintain mitochondrial integrity. It is part of the endoplasmic reticulum-associated degradation (ERAD) complex that ensures the removal of misfolded proteins. Without USP30's activity cells may accumulate damaged proteins leading to cellular stress and dysfunction. This activity influences mitochondrial dynamics by counteracting the ubiquitination activities within cells.
Pathways
USP30 plays a significant role in the mitochondrial quality control pathway and the ubiquitin-proteasome pathway. It regulates Parkin-mediated mitophagy by removing ubiquitin from mitochondrial substrates impacting processes that involve protein turnover and energy balance. USP30 works in conjunction with proteins like Parkin and PINK1 to maintain mitochondrial health by controlling the balance between ubiquitination and deubiquitination.
Deficiencies of USP30 associate with neurodegenerative diseases like Parkinson’s disease where impaired mitophagy and protein accumulation occur. It also connects with mitochondrial disorders as improper regulation of mitochondrial proteins by USP30 leads to energy production issues. In these conditions USP30's interaction with proteins Parkin and PINK1 becomes important for preventing cellular and mitochondrial dysfunction.