Abcam, ab314812, Alexa Fluor® 647 Anti-KLRF1 antibody [EPR23633-150]

Size: 100µL
Rabbit Recombinant Monoclonal KLRF1 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23633-150,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KLRF1 also known as CD314 or NKp80 is a receptor protein found primarily on natural killer (NK) cells with a significant mass of about 30 kDa. Researchers have identified its expression mainly in tissues rich in NK cells such as the spleen and peripheral blood. This receptor structurally belongs to the C-type lectin-like domain family and plays an important role in the immune response by binding to the CLEC2B ligand on target cells facilitating NK cell activation and cytotoxicity.
Biological function summary
In immune surveillance and homeostasis KLRF1 acts as an important activating receptor on natural killer cells. It does not form a complex but works independently to control cellular immune responses by recognizing stress-induced ligands on target cells. Upon interaction with its ligand KLRF1 triggers a cascade of intracellular signals that promote cytotoxic activity and cytokine secretion contributing to the elimination of virally infected or transformed cells.
Pathways
In the context of innate immune response signaling KLRF1 interacts with pathways involving NK cell-mediated cytotoxicity. KLRF1 engagement stimulates pathways similar to those activated by other receptors in the natural killer cell repertoire such as KLRK1 (NKG2D). These interactions play roles in maintaining balance within the immune system by modulating NK cell activity and ensuring efficient target cell elimination.
KLRF1 has implications in immune system-related conditions such as cancers and viral infections. Altered expression or function of KLRF1 may contribute to the escape of tumor cells from immune surveillance or decreased viral clearance. Associations with proteins like KLRD1 (CD94) in certain hematological malignancies suggest a role for KLRF1 in disease pathogenesis where NK cell dysfunction is present underlining its significance in developing therapeutic interventions.