Abcam, ab315010, Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free

Size: 100µg / 1mg
Knockout Tested Rabbit Recombinant Monoclonal ATG14L antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26188-66,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:In western blot we detect a double banding pattern. Based on the location of the immunogen, this antibody recognises both ATG14L and ATG14S isoforms (PMID: 36371383).

Product details:
ab315010 is the carrier-free version of
ab315009
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATG14 also known as Barkor is an important component involved in autophagy a cellular process for degrading and recycling cellular components. The protein has an approximate mass of 55 kDa. Researchers have observed ATG14 expression in various tissues with particularly high levels in the brain and liver. It plays a significant role in the formation and maturation of autophagosomes which are double-membrane structures that capture unnecessary cellular material for degradation.
Biological function summary
ATG14 serves as an important regulator within the autophagy machinery. It functions as a part of the Beclin-1-VPS34 complex which is essential for initiating the nucleation of the autophagosome membrane. This complex facilitates the localization of other autophagy-related proteins to the pre-autophagosomal structures thereby aiding in assembling the autophagosome. Through its ability to coordinate this assembly ATG14 influences both cellular homeostasis and response to stress.
Pathways
Researchers place ATG14 at a central point in the autophagy signaling pathway. It has connections to the PI3K signaling pathway where it interacts with Beclin-1 and VPS34 proteins. ATG14's function ties into the nutrient-sensing pathways that regulate cell growth and metabolism particularly under conditions of cellular stress or nutrient deprivation. This involvement reveals its relevance to cellular adaptive mechanisms which are important for organisms to maintain balance under varying environmental conditions.
ATG14 associates with neurodegenerative diseases and certain types of cancer. Its role in autophagy suggests influence on the development and progression of neurodegenerative disorders like Alzheimer's disease where malfunctioning autophagy leads to the accumulation of defective proteins. Furthermore aberrations in autophagy regulated by ATG14 may contribute to cancer progression through interactions with oncogenes and tumor suppressors such as Beclin-1. These associations underline ATG14’s potential as a target for therapeutic strategies in treating related diseases.