Abcam, ab315296, SARS-CoV2 Nucleoprotein ELISA Kit

Size: 1 x 96Tests
SARS-CoV2 Nucleoprotein ELISA Kit is a single-wash 90-min Simplestep used to quantify SARS-CoV2 Nucleoprotein with a sensitivity of 17.045 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Serum, EDTA Plasma, Saliva, Cell culture media,
Reacts with:SARS-CoV-2,
Assay type:Sandwich (quantitative),
Sensitivity:= 17.045 pg/mL,
Range:46.875 - 3000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
SARS-CoV2 Nucleoprotein SimpleStep ELISA
kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SARS-CoV2 Nucleoprotein protein in human serum, plasma, saliva and cell culture supernatant. Quantitate SARS-CoV2 Nucleoprotein with 17.045 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab315065
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The nucleocapsid protein of SARS-CoV-2 also known as N protein or COVID-19 nucleocapsid protein is important for the viral replication and packaging processes. It has a mass of approximately 43-46 kDa and is expressed in the cytoplasm of infected host cells. This protein is highly conserved among coronaviruses and plays a significant role in the viral lifecycle by binding to the viral RNA genome thereby facilitating its encapsulation within the virion. Moreover it aids in stabilizing the viral RNA structure and is essential for ribonucleoprotein complex formation.
Biological function summary
The nucleocapsid protein of SARS-CoV-2 interacts directly with other viral components and host cellular machinery to orchestrate efficient viral assembly and release. It forms a ribonucleoprotein complex by self-association and binding to the RNA genome promoting virion integrity. This protein also plays a part in modulating host cell responses potentially by interfering with antiviral signaling pathways ensuring that the virus evades the host immune system to some degree.
Pathways
Many scientific studies show that the SARS-CoV-2 nucleocapsid protein is involved in the viral replication and assembly pathways. It interacts with viral proteins such as the membrane (M) protein and possibly others to facilitate these processes. The protein also exhibits connectivity with cellular pathways related to RNA processing and metabolism due to its RNA-binding capabilities further influencing viral replication efficiency and host-pathogen interactions.
The nucleocapsid protein has a significant association with COVID-19 the disease caused by SARS-CoV-2. Its expression correlates with viral pathogenicity and immune response dysregulation which can lead to severe outcomes in infected individuals. The N protein has links with comorbid conditions potentially affecting disease progression. Furthermore the nucleocapsid protein interacts with host proteins like G3BP1 and other cellular factors that may contribute to immune modulation influencing the clinical symptoms associated with COVID-19.