Abcam, ab315800, Anti-CYP51A1/CYP51 antibody [EPR27379-150]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal CYP51A1/CYP51 antibody. Suitable for mIHC, IHC-P, WB, Flow Cyt (Intra), IP, ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27379-150,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:Flow Cyt (Intra), WB, IHC-P, IP, mIHC, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CYP51A1 also known as CYP51 is a cytochrome P450 enzyme. It plays an important mechanical role in cholesterol biosynthesis specifically catalyzing the demethylation of lanosterol. This enzyme has a molecular mass of approximately 55 kDa. CYP51A1 is expressed in multiple tissues including the liver testes and adrenal glands reflecting its widespread functional importance within the body.
Biological function summary
CYP51A1 is essential in the production of sterols such as cholesterol which are fundamental components of cell membranes. It does not operate alone; rather it forms part of an enzymatic complex involved in lipid homeostasis. By participating in this complex CYP51A1 influences cell membrane integrity fluidity and signaling.
Pathways
The activity of CYP51A1 integrates into the cholesterol biosynthesis pathway where it serves a critical role in the downstream conversion of lanosterol to cholesterol. Its action influences the pathway alongside other key enzymes like HMG-CoA reductase. Additionally the enzyme participates in broader lipid metabolic pathways linking with proteins such as squalene synthase that also contribute to sterol production.
CYP51A1's activity associates with certain lipid metabolism disorders including congenital adrenal hyperplasia and Smith-Lemli-Opitz syndrome. These conditions often involve disrupted cholesterol synthesis where CYP51A1’s malfunction can play a part. Moreover CYP51A1 connects to liver function through its interaction with proteins such as sterol regulatory element binding proteins (SREBPs) which regulate cholesterol homeostasis and can be associated with liver diseases.