Abcam, ab315915, Anti-Quiescin Q6 antibody [EPR25653-17] - BSA and Azide free (Capture)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Quiescin Q6 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25653-17,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab315915 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Quiescin Q6. The recommended pair for sandwich ELISA is:
Capture: ab315915, Human Quiescin Q6 Capture Antibody (unconjugated)
Detector:
ab315916
, Human Quiescin Q6 Detector Antibody (unconjugated)
The reference range value is 31.25-2000 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Quiescin Q6 also known as QSOX1 is an enzyme belonging to the Quiescin-sulfhydryl oxidase family. It functions by catalyzing the oxidation of sulfhydryl groups to form disulfide bonds in proteins an important step for proper protein folding. QSOX1 has a molecular mass between 60-100 kDa depending on its isoform. This protein is expressed in various tissues with high levels present in the pancreas placenta and some cancer cells suggesting a role in tissue specific processes and during certain pathological conditions.
Biological function summary
Quiescin Q6 influences cell growth and differentiation. The protein is involved in extracellular matrix organization where it plays a role in maintaining the stability and function of connective tissues. It doesn't typically assemble into large complexes but interacts with substrates directly facilitating the formation of stable proteins. QSOX1 ensures that proteins achieve their functional conformation by reshaping their disulfide bonds efficiently aiding in the cellular machinery of protein conformation.
Pathways
The functions of Quiescin Q6 intersect with the protein folding and oxidative stress response pathways. It relates closely to the endoplasmic reticulum protein folding pathway due to its role in disulfide bonding as well as to the antioxidant defense pathway where it mediates protection against oxidative stress. Its activity connects with proteins such as protein disulfide isomerase (PDI) which also participate in creating disulfide bonds but at different stages or cellular locations showcasing distinct but cooperative functions within these pathways.
Quiescin Q6 has been linked to cancer progression and fibrosis. Its overexpression has been observed in various tumors indicating a potential role in tumor growth and metastasis. In fibrotic diseases elevated levels of QSOX1 contribute to excessive extracellular matrix deposition. It connects with the transforming growth factor-beta (TGF-beta) pathway a critical regulator in fibrosis and cancer where QSOX1's regulation of matrix composition may influence TGF-beta signaling. Understanding these connections offers potential for developing targeted therapeutic strategies in treating related diseases.