Abcam, ab316437, Recombinant Mouse NT-ProANP Protein Standard

Size: 10µg
Recombinant Mouse NT-ProANP Protein Standard is a Mouse Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.
Key facts
Purity:>80% SDS-PAGE,
Expression system:HEK 293 cells,
Tags:Tag free,
Applications:sELISA, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P05125,
Animal free:Yes,
Carrier free:No,
Species:Mouse,
Storage buffer:pH: 7.3 - 7.5Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Product details:
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The proANP also known as N-terminal pro-atrial natriuretic peptide (NT-proANP) is a biochemical precursor involved in the synthesis of atrial natriuretic peptide (ANP). This peptide is expressed mainly in the cardiac atrial myocytes. proANP has a molecular mass of approximately 28 kDa. The heart releases it in response to atrial stretch due to increased blood volume assisting the body in managing vascular homeostasis.
Biological function summary
NT-proANP participates in fluid balance and blood pressure regulation. It is part of a complex system involved in natriuresis which promotes sodium excretion via urine therefore reducing blood volume and pressure. ANP the active hormone plays a large role in vasodilation contributing to cardiovascular health by decreasing peripheral resistance and reducing cardiac workload.
Pathways
ProANP's role extends into the renin-angiotensin-aldosterone system (RAAS) and the natriuretic peptide system. Within these pathways NT-proANP acts to counterbalance the effects of angiotensin II and aldosterone which increase blood pressure and fluid retention. It has a significant relationship with protein NPPB also known as brain natriuretic peptide (BNP) both of which function to support cardiovascular stability and electrolyte balance.
NT-proANP has connections to conditions like heart failure and hypertension. Elevated levels of NT-proANP may indicate heart failure as the heart releases more peptides in response to increased atrial pressure. NT-proANP's relationship with NPPB is clinically useful as both markers are used to diagnose and monitor heart failure. Their combined expression offers insights into cardiac function and the efficacy of therapeutic interventions.