Abcam, ab316910, Human CD16 ELISA Kit- Intracellular

Size: 1 x 96Tests
Human CD16 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human CD16 with a sensitivity of 5.717 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.717 pg/mL,
Range:19.531 - 1250 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CD16 SimpleStep ELISA
kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD16 protein in Tissue extracts. Quantitate Human CD16 with 5.717 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD16 often known as Fc gamma receptor III (FcγRIII) comes in two isoforms: CD16a and CD16b. CD16a is a transmembrane protein with a mass of about 50-65 kDa while CD16b a glycosylphosphatidylinositol (GPI)-anchored receptor holds a mass near 48-61 kDa. CD16 is expressed on natural killer (NK) cells monocytes macrophages and neutrophils depending on the variant. It serves as a low-affinity receptor for the Fc portion of IgG antibodies mediating essential immune functions like antibody-dependent cellular cytotoxicity (ADCC).
Biological function summary
CD16 plays an important role in immune response regulation and is part of the larger IgG receptor family. As a member of the receptor complex CD16 enables effector cells to identify and eliminate IgG-bound pathogens or diseased cells. The differential expression of CD16a and CD16b provides distinct roles in immune signaling. CD16a expressed on NK cells is involved in ADCC by enabling these cells to recognize and destroy antibody-coated target cells. CD16b predominantly found on neutrophils influences the phagocytosis process and aids in inflammatory response modulation.
Pathways
CD16 integrates into significant immune response pathways. In the ADCC pathway CD16a interacts with other proteins like FcεRI gamma-chain to trigger cytotoxic activities in NK cells. The CD16b isoform participates in neutrophil-mediated immune responses connecting to downstream signaling molecules that modulate inflammation and pathogen clearance. These interactions ensure an effective immune response and maintain homeostasis by removing pathogenic threats.
CD16 plays vital roles in immune-related conditions like autoimmune diseases and chronic inflammation. Aberrant CD16 expression or function is associated with autoimmune conditions such as rheumatoid arthritis where altered CD16a and CD16b expressions contribute to the pathology. Furthermore CD16 links to systemic lupus erythematosus (SLE) interacting with proteins like complement factors C3 and C4 influencing disease progression and severity. Understanding CD16's role offers insights into these disorders and informs potential therapeutic strategies.