Abcam, ab316973, Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91]

Size: 10µL-TRIAL / 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal ompF antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP, I-ELISA, IHC-P and reacts with Transfected cell lysate - Pseudomonas aeruginosa, Transfected cell line - Pseudomonas aeruginosa, Recombinant full length protein - Pseudomonas aeruginosa samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28629-91,
Isotype:IgG,
Carrier free:No,
Reacts with:Pseudomonas aeruginosa,
Applications:IP, I-ELISA, IHC-P, ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pseudomonas Outer Membrane Porin F (OprF) is a protein in Pseudomonas aeruginosa with a molecular mass of approximately 38 kDa. It forms channels across the bacterial outer membrane and allows small hydrophilic molecules to pass functioning as a non-specific diffusion channel. OprF is essential for maintaining the structural integrity of the outer membrane and it is majorly expressed in the outer membrane of Pseudomonas species.
Biological function summary
The Pseudomonas Outer Membrane Porin F plays an important role in nutrient uptake and waste removal. As a part of the outer membrane complex OprF works closely with other porins facilitating the transport of ions and small molecules. It contributes to the permeability of the bacterial membrane which is critical for the bacteria's adaptability and survival in diverse environments.
Pathways
OprF is significant in the general porin-mediated transport pathways that facilitate the exchange of essential nutrients and ions. OprF interacts with other porin proteins forming part of a porous barrier that aligns with the bacteria's need for environmental adaptation. Its role is critical in the context of biofilm formation pathways and antibiotic resistance mechanisms often partnering with OprD porin in these processes.
OprF is linked with chronic infections particularly in cystic fibrosis patients where Pseudomonas aeruginosa displays increased pathogenicity. OprF interacts with proteins like AlgC promoting biofilm formation that contributes to the bacterium's resistance to treatment. Its involvement in multidrug resistance also aligns with its role in chronic lung infections complicating the treatment strategies for affected individuals.