Abcam, ab316996, Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human beta 2 Defensin/BD-2.
Key facts
Reacts with:Human,
Assay type:ELISA set,
Range:1.56 - 100 pg/mL,
Assay Platform:Reagents

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta 2 defensin also known as BD-2 or beta-defensin 2 is a small antimicrobial peptide with a molecular mass of about 4.6 kDa. It plays an important role in the innate immune response by disrupting microbial membranes. BD-2 is expressed in epithelial cells making it important for creating a protective barrier in the skin respiratory tract and urogenital tract. This peptide acts directly against bacteria fungi and some viruses helping to maintain microbial homeostasis in these areas.
Biological function summary
Beta 2 defensin serves as a critical component of host defense mechanisms. It not only shows antimicrobial properties but also influences inflammatory processes. BD-2 interacts with immune cells recruiting and activating them for enhanced defense response. Presently there is no evidence of beta 2 defensin being part of a larger protein complex focusing its function on immediate immune response and cellular signaling in areas it is expressed.
Pathways
Beta 2 defensin engages in critical pathways of immune protection. It is part of the toll-like receptor signaling pathway and engages the NF-kB pathway. These pathways are essential for activating immune responses when pathogens are recognized. Additionally beta 2 defensin interacts with other defensins and related antimicrobial peptides to mount an effective immune response coordinating with various cytokines and chemokines to balance protective actions.
Beta 2 defensin has connections to inflammatory and autoimmune conditions. It has a potential link to psoriasis where its expression levels are increased serving both protective and inflammatory roles. BD-2 is also investigated in cystic fibrosis where its impaired function can contribute to chronic lung infections. In these conditions the altered balance of BD-2 associates it with proteins involved in immune dysregulation contributing to the pathophysiology of these disorders.