Abcam, ab317206, Anti-SARS-CoV-2 (COVID-19) nsp3 / PL-pro antibody [HL2350]

Size: 100µL
Rabbit Monoclonal R1A antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - SARS-CoV-2, Transfected cell line - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL2350,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS-CoV-2 nonstructural protein 3 commonly known as nsp3 plays an important role in the replication-transcription complex of the virus. Its mass is approximately 213 kDa. This multi-functional protein is expressed in infected host cells where it is involved in processes essential for viral replication. Nsp3 includes several functional domains such as a papain-like protease and ADP-ribose-1ʹ-phosphatase enabling it to process viral polypeptides and interfere with host cellular processes.
Biological function summary
Nsp3 is critical in the formation of the replication complex where it collaborates with other nonstructural proteins like nsp4 and nsp6. These interactions facilitate the modification of host cell membranes creating a conducive environment for viral RNA synthesis. In addition nsp3's enzymatic activity is necessary for inhibiting host immune responses supporting the virus's ability to establish infection within a host.
Pathways
Nsp3 participates in the viral life cycle by influencing key pathways including the autophagy pathway and translation initiation. Nsp3 can modulate the host's autophagy which typically helps in degrading cellular components to favor viral replication. Additionally nsp3 interferes with translation initiation affecting the host's ability to synthesize proteins therefore aiding in the initiation of viral protein synthesis. It cooperates with proteins like nsp5 and nsp12 in these pathways further ensuring efficient production of viral components.
Nsp3 is directly linked to COVID-19 the disease caused by SARS-CoV-2. This protein's ability to manipulate the host immune response highlights its significance in exacerbating the inflammatory response seen in severe cases. Nsp3 also connects to cytokines which are elevated during hyperinflammatory responses contributing to the pathophysiology of the disease. Additional insights into nsp3's functions can aid in the development of therapeutic strategies targeting COVID-19.