Abcam, ab317253, Anti-ATAD3A antibody [EPR27484-26] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ATAD3A antibody. Carrier free. Suitable for WB, IP, IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Recombinant fragment - Human, Transfected cell lysate - Human, Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27484-26,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, IP, IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Not suitable for mouse and rat IHC-P. This antibody recognizes overexpressed human ATAD3A isoform 2 and isoform 1 (UniProt ID: Q9NVI7). It may detect endogenous levels of ATAD3 isoform 1.

Product details:
ab317253 is the carrirer-free version of
ab317252
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATAD3A also known as ATPase family AAA domain-containing protein 3A functions as a mitochondrial membrane protein with an approximate molecular mass of 67 kDa. This protein is expressed widely in human tissues with high levels detected in the brain heart and liver. Mechanically ATAD3A facilitates interactions between the inner and outer mitochondrial membranes playing a role in mitochondrial dynamics and integrity.
Biological function summary
The maintenance of mitochondrial structure and function heavily involves ATAD3A. It forms part of a multiprotein complex that ensures the proper organization of mitochondrial nucleoids thereby regulating mitochondrial DNA replication and transcription. It also participates in maintaining the stability and distribution of mitochondrial proteins contributing to the overall energy metabolism of the cell.
Pathways
ATAD3A engages in key processes related to mitochondrial biogenesis and dynamics. Involvement in pathways like the mitochondrial DNA replication pathway and the energy production pathway highlights its role in maintaining cellular homeostasis. The protein interacts with others including DRP1 and MFN2 essential proteins that regulate mitochondrial fission and fusion processes contributing to its role in energy metabolism.
ATAD3A has associations with several conditions affecting mitochondrial function. Mutations or dysregulation in ATAD3A contribute to neurodegenerative disorders and certain types of cancer. It has been linked to Parkinson's disease due to its influence on mitochondrial homeostasis and is connected with Alzheimer's disease through its interactions with proteins like PINK1 another critical player in mitochondrial maintenance and function.