Abcam, ab317528, Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401]

Size: 100µL
Rabbit Recombinant Monoclonal ATP6V1B1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16401,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP6V1B1 and ATP6V1B2 are subunits of the V-type proton ATPase (vacuolar ATPase V-ATPase) important for proton translocation across intracellular membranes. Known also as ATP synthase subunits B1 and B2 these proteins together with other subunits form part of the cytoplasmic V1 domain of the enzyme. ATP6V1B1 has a molecular mass of approximately 56 kDa while ATP6V1B2 is slightly larger at around 57 kDa. Both subunits are widely expressed in various tissues but are specifically abundant in the kidney and inner ear.
Biological function summary
ATP6V1B1 and ATP6V1B2 participate in acidification of intracellular environments a fundamental process for cellular homeostasis and function. They are integral components of the V-ATPase complex which actively transports protons into lysosomes endosomes and other vesicles generating acidic conditions essential for protein degradation vesicular trafficking and hormone activation. This acidification is also important for maintaining the optimal pH necessary for various enzymatic activities within organelles.
Pathways
These subunits play an important role in the regulation of the mTOR signaling pathway and endocytosis pathway both critical for cell growth proliferation and nutrient sensing. The activity of V-ATPase influences these pathways by affecting pH-dependent processes required for receptor-mediated endocytosis and nutrient availability. ATP6V1B1 and ATP6V1B2 interact with other V-ATPase subunits such as ATP6V1A and ATP6V1C to modulate their function in these pathways.
Mutations and dysfunctions in ATP6V1B1 and ATP6V1B2 are linked to distal renal tubular acidosis (dRTA) and sensorineural hearing loss. These conditions result from altered proton transport and subsequent disruptions in acid-base balance. The relationship with dRTA involves the defective acidification of urine a process dependent on V-ATPase activity. Additionally altered function of these subunits has implications for their interaction with other proton pumps and transport proteins such as the carbonic anhydrase II which work together in maintaining pH equilibrium in the kidney and inner ear.