Abcam, ab317612, Anti-Giantin antibody [EPR27334-4] - Golgi Marker

Size: 10µL-TRIAL / 20µL / 100µL / 1mL
Knockout Tested Rabbit Recombinant Monoclonal Giantin antibody. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27334-4,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Giantin also known as Giantin Golgi or Giantin protein is a large coiled-coil protein with a molecular mass of approximately 376 kDa. It localizes primarily to the cis/medial region of the Golgi apparatus serving as a Golgi marker. As a part of the structural framework of the Golgi it plays an important role in maintaining the integrity and architecture of the Golgi stacks. Giantin is highly expressed in a variety of cell types including those in the liver kidney and brain making it an important target in cell biology studies.
Biological function summary
The Giantin protein functions as a scaffold that supports the Golgi apparatus’s dynamic organization. It is part of a larger complex that influences vesicular trafficking within the cell. This ability to interact with various vesicle coat proteins points to its important role in the sorting and transport of proteins and lipids through the secretory pathway. Besides Giantin is involved in the assembly of the Golgi matrix and influences its disassembly during mitosis suggesting a significant involvement in cell proliferation and division.
Pathways
Giantin helps regulate intracellular transport pathways connecting the endoplasmic reticulum (ER) and Golgi as well as intra-Golgi trafficking. It interfaces with other significant proteins such as GM130 and GRASP65 which are involved in Golgi dynamics and structure. This protein also participates in the coordination of the secretory pathway important for the modification and sorting of proteins destined for the cell surface or lysosomes.
Giantin has been linked to conditions involving impaired protein glycosylation and Golgi-related transport defects. Notably defects in Giantin function are associated with connective tissue disorders and certain forms of Golgi dysfunctions like Congenital Disorders of Glycosylation (CDG). Additionally Giantin’s interactions with proteins such as Sec23/24 and Bet3 which are cargo-binding components of the COPII vesicle coat connect its functional disruption to diseases characterized by impaired vesicular trafficking and cellular secretion malfunctions.