Abcam, ab318264, Anti-Nogo A + Nogo D antibody [EPR26286-15]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal RTN4 antibody. Suitable for WB, IHC-P, IHC-Fr, ICC/IF and reacts with Transfected cell lysate - Mouse, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26286-15,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IHC-P, WB, IHC-Fr, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Very weak non-specific staining is detected in mouse spleen and kidney tissue, ab315792 is recommended as alternative for mouse IHC.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nogo A also known as Reticulon 4-A (RTN4-A) and Nogo D are part of the Nogo protein family. Nogo A is a 200 kDa protein while Nogo D is smaller around 37 kDa. These proteins are mainly expressed in the central nervous system particularly in the oligodendrocytes and neurons. Mechanically they are involved in the inhibition of axonal growth and neural regeneration functioning as a myelin-associated protein. The protein structure consists of three main regions: N-terminal central domain and a long C-terminal domain each contributing differently to its inhibitory functions.
Biological function summary
Nogo A and Nogo D are important for maintaining neural network stability. They belong to the larger reticulon protein family which forms part of the endoplasmic reticulum network. Nogo A binds to specific receptors such as Nogo receptor 1 (NgR1) to mediate growth inhibition. This interaction causes a signaling cascade that limits neuronal sprouting and potentially stabilizes synapses. Nogo D’s biological role is less defined but suggests involvement in modulating similar pathways as Nogo A.
Pathways
Nogo A plays a significant role in neural inhibition and recovery processes. It integrates into the RhoA/ROCK signaling pathway which influences cytoskeletal dynamics and axonal growth inhibition. This protein works with others like RhoA GTPase within this pathway to execute its functions. Additionally Nogo A interacts with MAG and OMgp proteins through shared receptors further impacting neuronal growth and recovery after injuries.
Nogo A and Nogo D have prominent roles in conditions such as multiple sclerosis (MS) and spinal cord injuries. Nogo A limits axonal regeneration and remyelination in MS often in interaction with myelin-associated inhibitors like MAG restricting neuronal recovery. In spinal cord injuries the presence of Nogo A is closely associated with limited neural repair connecting it with proteins part of the glial scar formation. These interactions highlight the potential of targeting Nogo proteins for therapeutic purposes in regenerative medicine.