Abcam, ab320045, Human Calprotectin (S100A8/S100A9) ELISA Kit

Size: 1 x 96Tests / 10 x 96Tests
Human Calprotectin (S100A8/S100A9) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Calprotectin (S100A8/S100A9) with a sensitivity of 13.624 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant, Urine, Saliva, Milk, Cell Lysate, Tissue Extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 13.624 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Calprotectin (S100A8/S100A9) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Calprotectin (S100A8/S100A9) protein in Serum, Cit plasma, EDTA Plasma, Cell culture supernatant, Urine, Saliva, Milk, and Cell Lysate. Quantitate Human Calprotectin (S100A8/S100A9) with 13.624 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
S100A9 and S100A8 also known as calprotectin or MRP8/14 when they form a complex together are small calcium-binding proteins with molecular masses of approximately 13 kDa and 11 kDa respectively. These proteins show high expression levels in neutrophils and monocytes serving important roles in the immune response. Both S100A9 and S100A8 display the ability to bind calcium ions changing their conformation and functional status. This calcium-induced structural change is essential for interacting with other proteins and cellular components.
Biological function summary
The S100A9 and S100A8 proteins regulate the inflammatory response by modulating immune cell activity. They function as heterodimers forming complexes that influence cell adhesion migration and the production of reactive oxygen species. Such activities are integral to both acute and chronic inflammation. The proteins achieve these effects by acting as antimicrobial agents and by regulating leukocyte recruitment to sites of infection or injury.
Pathways
S100A9 and S100A8 participate in key inflammation- and immune-related signaling cascades such as the NF-κB pathway and the TLR4 signaling pathway. In these contexts they interact with associated proteins like TLR4 and RAGE (Receptor for Advanced Glycation End-products). Their involvement in these pathways highlights their role in amplifying inflammatory signals during immune responses promoting the expression of pro-inflammatory cytokines.
S100A9 and S100A8 links with conditions like rheumatoid arthritis and inflammatory bowel disease. The upregulation of these proteins often correlates with increased inflammation and severity in such diseases. Moreover they connect with joint damage observed in rheumatoid arthritis through their interaction with RAGE. In inflammatory bowel disease their elevated levels serve as biomarkers for disease activity and guide therapeutic strategies aimed at modulating the immune response.