Abcam, ab320646, Anti-ID2 antibody [EPR29025-9]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal ID2 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR29025-9,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody does not cross-react with overexpressed human ID1 (#P41134) by Western Blot.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ID2 also known as inhibitor of DNA binding 2 is a nuclear protein with a molecular weight approximately 15 kDa. It functions as a dominant negative regulator and lacks a DNA-binding domain but it forms heterodimers with basic helix-loop-helix (bHLH) transcription factors. This interaction with bHLH proteins inhibits their ability to bind DNA and initiate transcription. ID2 expresses in various tissues particularly in places with high cellular proliferation such as the nervous system and developing tissues.
Biological function summary
ID2 plays a role in cellular proliferation differentiation and development by modulating the activity of other transcription factors. It acts as part of a regulatory complex that blocks differentiation pathways allowing cells to maintain a proliferative and less differentiated state. ID2 often interacts with proteins like E2A and MyoD which are important for muscle cell differentiation. By regulating these factors ID2 influences the balance between cell growth and terminal differentiation.
Pathways
ID2 integrates into key signaling pathways particularly the TGF-beta and BMP signaling pathways. Through its interaction with the aforementioned bHLH transcription factors ID2 indirectly influences processes governed by these pathways. TGF-beta pathway for instance regulates cell growth and differentiation in a context-dependent manner incorporating ID2's regulatory effect on differentiation-suppressing transcription factors.
ID2 has implications in cancer and neurodegenerative diseases. Dysregulation of ID2 expression or function can contribute to tumorigenesis as it affects cell cycle progression and prevents differentiation both important in cancer development. Additionally ID2 has connections to proteins like pRb in retinoblastoma where it influences the pathway that leads to uncontrolled cellular proliferation. In the context of neurodegenerative disorders altered ID2 activity might impact neuronal differentiation providing links to cognitive deficits.